HORSE TIMES Magazine :: THE LEADING EQUESTRIAN MAGAZINE IN THE MIDDLE EAST
Add HorseTimes Egypt's Equestrian Magazine to your Favorites Join HorseTimes Egypt's Equestrian Magazine on Facebook Subscribe to HorseTimes Egypt's Equestrian Magazine on Youtube Follow HorseTimes Egypt's Equestrian Magazine on Google+ Pin HorseTimes Egypt's Equestrian Magazine Photos Follow HorseTimes Egypt's Equestrian Magazine Latest News
HOME PROFILE ADVERTISE SUBSCRIBE LINKS CONTACT

The Leading Equestrian Magazine In The Middle East

Welcome To Horse Times
HORSE TIMES is a complimentary quarterly English-language magazine established in Egypt in 1997. It is the only English language publication in the Middle East dedicated to improving access .. read more..

Horse Times Magazine :: Spring Issue # 53

PUBLISHED SPRING 2017, ISSUE 53 FEATURES:

20TH ANNIVERSARY
HRH PRINCESS HAYA BINT AL HUSSEIN

20TH ANNIVERSARY
RIDERS’ PROFILES

20TH ANNIVERSARY
BLUE BLOOD ADDICTED TO HORSES

20TH ANNIVERSARY
BEHIND THE SCENES

20TH ANNIVERSARY
CELEBRITIES WITH PASSION FOR HORSES

20TH ANNIVERSAR..

VIEW MAGAZINE ISSUES HERE >>

Horse Times Weekly Headlines :: Issue # 25

- MICHAEL JUNG RE-WRITES HISTORY TO BE CROWNED WINNER OF THE ROLEX KENTUCKY THREE-DAY EVENT FOR THE THIRD TIME

- ITALY’S LORENZO DE LUCA CLAIMS LGCT GRAND PRIX TITLE IN SHANGHAI

- TEAM GERMANY WINS THRILLING FEI NATIONS CUP™ JUMPING DIVISION 1 OPENER IN LUMMEN

- EMANUELE GAUDIANO COMPLETES ..

VIEW HEADLINES ISSUES HERE >>

HORSE TIMES RELEASES A NEW BOOK


ALLTECH FEI WORLD EQUESTRIAN GAMES
NORWAY 2014

Download here >>
NEWS EVENTS HEADLINES ISSUES ARTICLES VIDEOS STREAMING BLOG
HORSE TIMES BLOG ARTICLES DETAILS
>> GENE EXPRESSION IN LAMINITIS
By: NICOLA KERBYSON, Equine Veterinary Journal | Date: Wednesday, September 24, 2014
Horse Times Egypt: Equestrian Magazine :Riders Blog :GENE EXPRESSION IN LAMINITIS Use of laser capture microdissection for the assessment of equine lamellar basal epithelial cell signalling in the early stages of laminitis.

BS Leise, MR Watts, S Roy, AS Yilmaz, H Alder and JK Belknap

http://tinyurl.com/p8zsvok

Laser capture microdissection allows specific cells to be dissected from a tissue sample under microscopic guidance. This study utilised this technology to allow a highly purified population of laminar basal epithelial cells to be collected from lamellar biopsies in a carbohydrate (CHO) induced laminitis model. Eight horses were subjected to carbohydrate overload, lamellar biopsies were taken from the left forefoot prior to administration of carbohydrates, to act as a control group. Further lamellar biopsies were taken from the other feet at the onset of fever (or 24 hours post CHO administration) and the onset of Obel grade 1 lameness (or 48 hours post CHO administration). Laser capture microdissection was then used to isolate laminar basal epithelial cells, RNA sequencing and profiling then allowed the difference in lamellar gene expression profiles to be compared before, during and after the onset of laminitis. Forty genes were expressed differently (22 up-regulated, 18 down-regulated) at the time of development of laminitis, compared with before. One hundred and seven genes were expressed differently at the time of development of Obel grade 1 lameness, compared with before CHO administration. Genes related to the inflammatory response and extracellular matrix regulation were significantly altered at the time of laminitis development, and onset of clinical signs.

The bottom line: Altered gene expression in lamellar basal epithelial cells during the development and onset of laminitis shows that this cell is an active participant in the structural failure of lamellae during clinical laminitis.

Photo: Equine Veterinary Journal 19 AUG 2014 DOI: 10.1111/evj.12283
http://onlinelibrary.wiley.com/doi/10.1111/evj.12283/full#evj12283-fig-0002

--Ends--

http://www.evj.co.uk/evj
COMMENTS



Copyright 2009 - 2017 © Horse Times. All rights reserved. powered by zanss.com