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HORSE TIMES is a complimentary quarterly English-language magazine established in Egypt in 1997. It is the only English language publication in the Middle East dedicated to improving access .. read more..

Horse Times Magazine :: Winter Issue # 52

PUBLISHED WINTER 2016, ISSUE 52 FEATURES:

THE MOROCCO ROYAL TOUR
TRILOGY OF THE CHAMPIONS

THE 9TH SALON DU CHEVAL D’EL JADIDA
CARRIES A MESSAGE OF REVIVAL TO THE TRADITIONAL EQUESTRIAN ART

JUMPER OF THE YEAR
THE STORY OF BERTRAM ALLEN, A YOUNG JOCKEY WITH EXQUISITE SKILLS

GERMANY TAKE..

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Horse Times Weekly Headlines :: Issue # 24

- FEI WORLD CUP™ VAULTING FINAL 2017: ITALY AND GERMANY TAKE HOME MALE, FEMALE AND PAS-DE-DEUX

- CHI ALSHAQAB 2017: SCOTT BRASH SCOTT BRASH PREVAILS AT THE CSI5* GRAND PRIX

- BOYD EXELL WINS HIS SEVENTH FEI WORLD CUP™ DRIVING TITLE

- ISABELL WERTH WINS IN GOTHENBURG

- VON ECKERMANN SNAT..

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HORSE TIMES RELEASES A NEW BOOK


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NORWAY 2014

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>> SAMPLE HANDLING AND ACTH CONCENTRATION
By: NICOLA KERBYSON, Equine Veterinary Journal | Date: Wednesday, December 17, 2014
Horse Times Egypt: Equestrian Magazine :Riders Blog :SAMPLE HANDLING AND ACTH CONCENTRATION The effects of sample handling and N-phenylmaleimide on concentration of adrenocorticotrophic hormone in equine plasma

DI Rendle, E Litchfield, S Gough, A Cowling and KJ Hughes

http://tinyurl.com/n8t3n6a

Adrenocorticotrophic hormone (ACTH) is frequently measured as a diagnostic aid in the detection of pituitary pars intermedia dysfunction (PPID). Delays in analysis of blood samples are frequent with this test as external laboratories are often involved in analysis; the effect of delays of up to 48 hours has not previously been measured. This study measured the effect of time, centrifugation vs. gravity separation and the addition of maleimide (a protease inhibitor) on ACTH concentration. Eight healthy horses and eight horses with PPID were recruited, baseline ACTH was measured immediately after sampling using a chemiluminescent assay. Samples taken from each horse were divided and subjected to the following procedures: centrifugation, centrifugation and addition of maleimide, or gravity separated and addition of maleimide. All samples were stored at 22°C. Each sample was then analysed at 4, 8, 24 and 48 hours post collection. There was no effect on the addition of maleimide, there was a significant reduction in ACTH concentration in horses with PPID between 4 and 8 hours post collection and a wider range of ACTH values were identified with increasing time in horses with PPID. The differences in ACTH concentration over time were not of the magnitude to be of clinical relevance and may be similar to the biological variation observed over a similar time period. There was no significant difference in ACTH concentrations in samples separated by gravity compared to centrifugation.

The bottom line: Although there is some variation in ACTH concentration over time this has not been shown to be clinically relevant and may only be of significance in horses which had borderline ACTH concentrations at the time of sampling. Method of separation and the addition of protease inhibitors had no effect on ACTH concentration.

Photo: Pituitary Pars Intermedia Dysfunction (PPID)

--Ends--

http://www.evj.co.uk/evj
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